|
The carpophores of a mushroom, Naematoloma fasciculare (Fr.) Karst. were extracted with 0.2N NaOH and the extract was dialyzed through visking tube. It was found to contain an antitumor activity against sarcoma 180 implanted in mice. The components of this aqueous extract were found to be a polysaccharide and a protein by color reactions including Anthrone and Lowry-Folin tests. The hydrolysis of the polysaccharide with 3% HCl-MeOH and trimethylsilylation of the hydrolysate yielded five monosaccharides, i.e., glucose, fructose, mannose, galactose and xylose, which were detected and analyzed by GLC. After hydrolysis of the protein with 6N HCl, 15 amino acids, including aspartic acid and glutamic acid, were detected and analyzed by an auto amino acid analyzer.
|